Application of qNMR. Part 2:Quantification of carminic acid in cochineal extract
Carminic acid is a main component of cochineal extract,
originally obtained by drying scale insects and
extracting them with ethanol or water.
The purity of test reagents of carminic acid and
that of food additives of cochineal extract are
examined by qNMR.
Fig.1 qNMR spectrum of carmic acid
Table Summary of sample information and results of qNMR analyses
Fig.2 Correlation beween qNMR purity and LC peak area
- Purities of carminic acid in reagents and cochineal extracts were determined by qNMR.
- Purities from qNMR were confirmed to have strong correlation with LC peak area.
⇒It was found that qNMR is a useful quanti-fication analysis for natural products, for which standard samples for quantification are not available.