Measurement of Vitamin C/ Ascorbate Radical by Using ESR

  • Summary


Vitamin C (V.C)/ L-Ascorbic Acid(Fig. 1(a))is one of the vitamins which are most-studied in recent years.  It is known as a water-soluble antioxidant and it is also used as an additive for foods.  It can be auto-oxidized easily to give Dehydro-ascorbic acid (DHA) radical.

Fig. 1 (a) Vitamin C (b) DHA radial
Fig. 1 (a) Vitamin C
(b) DHA radial

The V.C. aqueous solution (1 Mol/L) was measured after N2-gas bubbling for 30 seconds.  The result is shown in Fig. 2.  For DHA radical, the unpaired electron is delocalized on the carbonyl groups C1 to C3, and it is expected to have considerable spin distribution on oxygen atoms 1).

図2 V.CラジカルのESRスペクトル
Fig.2  ESR Spectrum of V.C Radical

The ESR signal of DHA radical is recorded in precise in Fig. 3. The hyperfine couplings, a4H=0.18mT and a6CH2=0.019mT, are typical for DHA radical.
図3 V.Cラジカルのスペクトルパターン
Fig. 3  ESR signal of DHA radical

It is expected that quantity of DHA radical indicates the redox condition around the radical.2).


1) G.P.Laroff J.Amer.Chem.Soc. 94 9062-9073 1972
2) G.R.Buettner Free Radical Biology & Medicine 14 49-55 1993


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