The DEPT/INEPT Tool is used to extract three sub-spectra of CH, CH2 and CH3 signals from a series of DEPT (Distortionless Enhancement by Polarization Transfer) experiments.
The DEPT experiment can be run as either DEPT-45, DEPT-90 and DEPT-135. The number corresponds to a flip angle of the 1H selection pulse.
The DEPT-45 detects signals of all protonated carbons, i.e. CH, CH2 and CH3, with the same phase (sign). The DEPT-90 gives only CH peaks. The DEPT-135 gives signals of all protonated carbons, but CH and CH3 signals are positive, while CH2 peaks are negative. The signals of quaternary carbons are absent in all the DEPT spectra. By combining the DEPT-45, DEPT-90 and DEPT-135 spectra, it is possible to determine multiplicity of each carbon signal.
The DEPT/INEPT Tool automatically adds/subtracts DEPT spectra for your convenience.
① Open DEPT-45, DEPT-90 and DEPT-135 data.
② Select Analyze－DEPT/INEPT Analysis to open the DEPT/INEPT Tool window.
③ Click the Select Geometry button in the DEPT/INEPT Tool window.
Note that the cursor has changed into the Finger symbol.
④ Select each DEPT spectrum with the cursor.
⑤ Click the Process button in the DEPT/INEPT Tool window.
The result of DEPT spectra analysis has been shown in a new Data Slate window.
★If you prefer to use your Data Slate window to display the result of the DEPT/INEPT Tool
follow these steps:
⑥ Click the New button in the DEPT/INEPT Tool window.
The cursor has changed into the Finger symbol.
⑦ Click the menu bar in the Data Slate window.
⑧ Click the Process button in the DEPT/INEPT Tool window.
The result of DEPT analysis has been displayed in your selected Data Slate window.
! The intensity of the DEPT signal is sensitive to the JCH value variation.
If your sample has rather large JCH Δ, the CH, CH2 and CH3 sub-spectra may contain residual signals (artefacts).
- Please see the PDF file for the additional information.
Another window opens when you click.