MALDI Application: Analysis of the Natural Organic Compound SAAF Using the TOF-TOF Option

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MSTips No.183 The analysis above was conducted in collaboration with the Mass Analysis Group, Department of Physics, Graduate School of Science, Osaka University.
We wish to express our thanks to Prof. Michio Murata of the Department of Biomolecular Sciences, Department of Physics, Graduate School of Science, Osaka University, for providing the SAAF samples.

Sperm activating and attracting factor (SAAF) is a naturally occurring substance produced by ascidians (sea squirts) to promote the fertilization process. Previously, the structure of this compound was characterized by high-energy collision induced dissociation (CID) using a traditional four sector tandem double focusing mass spectrometer.1 In this work, we analyzed the SAAF from a ciona intestinalis by using a JMS-S3000 SpiralTOF™ equipped with the optional TOF-TOF analyzer to generate a high energy CID product ion spectrum for this compound.

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