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SpiralTOF-TOF Analysis of Bovine Serum Albumin [MALDI Application]

MSTips No.174

The JMS-S3000 “SpiralTOF™” is a MALDI-TOFMS that incorporates an innovative SpiralTOF™ ion optics system. The JMS-S3000 is available with a TOF-TOF option that can acquire high-energy collision induced dissociation (CID) product ion spectra for monoisotopically selected precursor ions. This monoisotopic precursor selection is made possible by the fact that the distance to the ion gate is 15 m, which is more than one order of magnitude longer than that of conventional MALDI TOF-TOF instruments. Additionally, the second TOF MS incorporates a re-acceleration mechanism and an offset parabolic reflectron, another innovative optical design developed by JEOL. This unique design enables seamless observation of product ions ranging from very low m/z up to that of the precursor ion. In a previous application note, we reported the analysis of bovine serum albumin (BSA) by Spiral and Linear modes. In this work, we show the analytical result for BSA by using the JMS-S3000 "SpiralTOF" with the TOF-TOF option.

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