• Features
  • Application
  • Related Products
  • Information

Features

EM-05500TGP TEM Tomograph System
3-dimensional Structure with high precision TEM Tomograph System is an automation system handling an entire process from an acquisition of sequential tilt images through to their 3-dimensional reconstruction. Its software (Recorder, Composer, Visualizer-Kai) employ unique algorithms for enabling the automation of various adjustments particularly required to Tomography.
 

Recorder

This software acquires sequential tilt images in a short time for Tomography. Many images of samples, taken in the course of continuous tilting with certain angle step, are necessary for accurate reconstruction. Operations such as sample tilting at a pre-set angle step, correction of view shift stemming from the tilt, adjustment of focusing and the acquisition of images are automatically carried out.

Recorder操作画面
Recorder Operation Screen
 

Composer

This software performs a 3-dimensional reconstruction from sequentially acquired tilt images. Crucial element required to the electron beam tomography is how to make an accurate alignment of the image data. This is resolved by calculation of 3-dimensional position on the tilt axis and analyzing the precise angle information, and as a result, a precise reconstruction in a short time with easy operation has been realized.
Composer
 

Visualizer-Kai

This software displays 3-dimensionally-reconstructed data by various methods such as volume rendering and surface rendering and also enables the intuitive observation on the screen as if handling by hands. By using a mouse, the images can be freely moved and displayed. The function of length measurement is also included.
Visualizer-Kai操作画面

Visualizer-Kai Operation Screen
Visualizer-Kai Operation Screen
 

High Tilt Sample Stage (optional)

To acquire image at high tilt angle, high tilt sample stage is needed.
高傾斜
 

Application Example of 3-dimensional Reconstruction: 3-dimensional structure analysis of a Tight junction


Measurement Condition
Accelerating Voltage: 120kV
Tilt angle: ±65°, 1° step
Sample: Tight junction (mouse’s small intestine epithelium, thickness 70nm)
Explanation: Tight junction is a cell adhesion structure existing in the top area of epithelium cell of vertebrata.

Tight junctionのTEM像
Fig. 1 TEM Image of Tight Junction

Tight junction works as a barrier against water or ions, as its cell membranes between neighboring cells closely adhere.  This time, we analyzed a 3-dimensional form of a cell membrane at the Tight junction by using EM-05500TGP. 


Movie 1: Positioning of image data by Composer
By using Composer, we adjusted the position of sequential tilt image photographed by Recorder. Although a fiducial marker such as an Au colloid particle was needed in conventional method, Composer realizes positioning without it.

Tight junctionのボリュームレンダリング像
Volume rendering image of tight junction


Movie2: volume-rendering image of tight junction
By using Visualizer-Kai the 3-dimensionally reconstructed image can be freely moved and observed.

Tight junctionのスライス像
Slice image of tight junction


Movie 3: slice image of tight junction
We can observe that the adhered structure of the cell membranes at a tight junction is a dynamic structure where adhering position changes 3-dimensionally, rather than the structure where membranes at the same position keep adhering.

波長
Length measurement

Visualizer-Kai has length-measurement function.
 

Specifications

Gallery

Related Products

Information

Installation Examples