- Transmission Electron Microscope (TEM)
- JEM-Z300FSC (CRYO ARM™ 300) Field Emission Cryo-Electron Microscope
JEM-Z300FSC (CRYO ARM™ 300) Field Emission Cryo-Electron Microscope
- Related Products
Automated specimen exchange system
The system is composed of a specimen stage to cool samples to liquid nitrogen temperature and a cryo-transfer system to automatically transfer the cooled samples to the cryo-stage. Liquid nitrogen is automatically supplied to the liquid nitrogen tank as required. This automated system features the storing of up to 12 samples and the exchange of an arbitrary one or more samples while the rest of the samples are kept cooled between the specimen stage and specimen exchange system.
Cold field emission gun (Cold FEG)
A Cold FEG produces a high-brightness electron beam with very small energy spread, offering high coherency. Thus, the CRYO ARM™ 300 achieves high resolution, high contrast imaging.
In-column Omega energy filter
Equipped with an in-column Omega energy filter, the CRYO ARM™ 300 acquires energy filtered images and energy loss spectra. Zero-loss images acquired with the microscope provide high contrast with reduced chromatic aberration.
Automated image acquisition software for Single Particle Analysis
The CRYO ARM™ 300 incorporates automated software. The software allows for automated detection of holes on the specimen grid for efficient acquisition of Single Particle Analysis images.
Hole-free phase plate *1
This unique phase plate is suitable for higher contrast in biological specimens that originally provide only low contrast.
Auto adjustment functions *2
Auto focus, auto coma-free alignment, auto parallel-beam illumination and other automated adjustments are available, enabling image acquisition under optimum conditions.
*1: Optional unit. *2: Images acquired with the bottom mount camera are used.
|Electron gun||Cold field emission gun (Cold FEG)|
|Energy filter||In-column Omega energy filter|
|Maximum specimen tilt angle||± 70°|
Specimen Stage / Automated specimen exchange system
Automated liquid-nitrogen filling system built-in
|Specimen cooling temperature||100K or less|
|Temperature measurement position||Specimen, Cryo-shield, LN2 tank|
|X、Y||Motor drive (movements: ±1 mm)
Piezoelectric elements (movements: ±0.5 μm)
|Z||Motor drive (movements: ±0.2 mm)|
|Tilt-X||Motor drive (tilts: ±70°)|
|Rotation within the specimen plane||0° or 90°|
|Specimen exchange system||Air-lock
Automated cryo-transfer system built-in
(specimen exchange chamber)
|105K or less|
|Specimen exchange cartridge||Up to 4 specimens can be changed at one time.|
|Specimen parking stage||Up to 12 specimens can be held.|
* Three-dimensional calibration image of β-galactosidase obtained using a cryo-electron microscope (CRYO ARM™ 200) with an accelerating voltage of 200 kV
β-galactosidase 2.6 Å resolution CRYO ARM™
β-galactosidase with PETG
CRYO ARM™ (Schottky 200 kV) / K2 summit
- Number of Images:
2,500 over 3 days by JADAS
- Image pixel size:
- Number of particle images:
350,000(Initial pickup), 88,564 (for final 3D reconstruction)
Motioncor2, Gctf, Gautomatch, Relion2.0
- Total dose:
70 e-/Å2 (70 frames (0.2 sec/frames x 14 sec)
Data: courtesy by Dr. T. Kato and Dr. K. Namba, Osaka University, August 2017