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Features

JEM-Z300FSC (CRYO ARM™ 300) Field Emission Cryo-Electron Microscope
The JEM-Z300FSC (CRYO ARM™ 300), equipped with a cold field emission gun, an in-column Omega energy filter, a side-entry liquid nitrogen cooling stage and an automated specimen exchange system, is a cryo-electron microscope (cryo-EM) that enables observation of bio-molecules at cryo-temperature. The automated specimen exchange system features the storing of up to 12 samples. In addition, the system allows for the exchange of an arbitrary one or more samples, thus enabling flexible scheduling. Furthermore, the combined use of a newly-designed in-column Omega energy filter and a Hole-free phase plate dramatically enhances the contrast of TEM images of biological specimens.

Automated specimen exchange system

The system is composed of a specimen stage to cool samples to liquid nitrogen temperature and a cryo-transfer system to automatically transfer the cooled samples to the cryo-stage. Liquid nitrogen is automatically supplied to the liquid nitrogen tank as required. This automated system features the storing of up to 12 samples and the exchange of an arbitrary one or more samples while the rest of the samples are kept cooled between the specimen stage and specimen exchange system.

Cold field emission gun (Cold FEG)

A Cold FEG produces a high-brightness electron beam with very small energy spread, offering high coherency. Thus, the CRYO ARM™ 300 achieves high resolution, high contrast imaging.

In-column Omega energy filter

Equipped with an in-column Omega energy filter, the CRYO ARM™ 300 acquires energy filtered images and energy loss spectra. Zero-loss images acquired with the microscope provide high contrast with reduced chromatic aberration.

Automated image acquisition software for Single Particle Analysis

The CRYO ARM™ 300 incorporates automated software. The software allows for automated detection of holes on the specimen grid for efficient acquisition of Single Particle Analysis images.

Hole-free phase plate *1

This unique phase plate is suitable for higher contrast in biological specimens that originally provide only low contrast.

Auto adjustment functions *2

Auto focus, auto coma-free alignment, auto parallel-beam illumination and other automated adjustments are available, enabling image acquisition under optimum conditions.

Link


*1: Optional unit. *2: Images acquired with the bottom mount camera are used.

Specifications

Main instrument

Electron gun Cold field emission gun (Cold FEG)
Accelerating voltage 300kV
Energy filter In-column Omega energy filter
Maximum specimen tilt angle ± 70°

Specimen Stage / Automated specimen exchange system

Specimen stage
Coolant Liquid nitrogen
Automated liquid-nitrogen filling system built-in
Specimen cooling temperature 100K or less
Temperature measurement position Specimen, Cryo-shield, LN2 tank
Specimen movements
X、Y Motor drive (movements: ±1 mm)
Piezoelectric elements (movements: ±0.5 μm)
Z Motor drive (movements: ±0.2 mm)
Tilt-X Motor drive (tilts: ±70°)
Rotation within the specimen plane 0° or  90°
Specimen exchange system Air-lock
Automated cryo-transfer system built-in
Cooling temperature
(specimen exchange chamber)
105K or less
Specimen exchange cartridge Up to 4 specimens can be changed at one time.
Specimen parking stage Up to 12 specimens can be held.

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