- Mass Spectrometer
- MALDI: JMS-S3000 SpiralTOF™-plus Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer
MALDI: JMS-S3000 SpiralTOF™-plus Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer
- Related Products
The JMS-S3000 is a MALDI-TOFMS that incorporates the innovative SpiralTOF ion optical system. The JMS-S3000 SpiralTOFTM has evolved into the SpiralTOF™-plus with much improved MS imaging functionality. The JMS-S3000 SpiralTOFTM-plus defines a new standard in MALDI-TOFMS performance and provides state-of-the-art analytical solutions for a wide range of research areas such as functional synthetic polymers, materials science, and biomolecules.
Setting the new standard in MALDI-TOFMS performance
A conventional reflectron TOFMS with delayed extraction method can only achieve high mass-resolving power over a limited mass range. The SpiralTOF™-plus overcomes this challenge by extending the flight distance, thus successfully achieving high mass-resolving power over a wide mass range.
Reduced topographic effect of matrix crystal
The topographic effect of the matrix crystal leads to a difference in flight start position for the ions, resulting in a difference in flight time. In the conventional ion optical system, this time difference degrades the mass resolving power and also the mass accuracy obtained with external mass calibration. With its extended flight distance, the JMS-S3000 reduces this effect to the minimum and achieves highly reproducible mass resolving power and high mass accuracy with an external mass calibration. High mass-resolution and mass accuracy can be maintained for imaging analysis of a biological specimen in which a large number of mass spectra are acquired across a large area and the specimen surface is likely to be uneven.
The SpiralTOF™-plus with TOF-TOF option is designed to take full advantage of high-energy collision-induced dissociation (HE-CID). The SpiralTOF™-plus 1st MS allows monoisotopic selection of precursor ions, which facilitates unambiguous interpretation of the information-rich HE-CID product ion mass spectra.
Linear TOF Option
The Linear TOF option allows for high-sensitivity analyses of high molecular weight samples (molecular mass: several tens of thousands or more) and samples that tend to undergo post source decay. Alternating the use of the Spiral and Linear modes enables a wider range of compounds to be analyzed efficiently.
msRepeatFinder: the Definitive Polymer Analysis Software
Compositional distribution of polymers with different end groups
msRepeatFinder (option) combines Kendrick Mass Defect (KMD) and Kendrick Mass Remainder (KMR) plots to visualize compositional distributions of homopolymers with different end groups. The program also calculates parameters related to molecular weight distributions by grouping and color coding each polymer series.
|Total ion intensity||Center of KMD value||Center of NKM value||Number average molecular weight (Mn)||Weight average molecular weight (Mw)||Polydispersity|
Analysis of copolymers
In mass spectral data of copolymers, numerous peaks with small mass differences are typically observed. Fraction Base KMD plots can clearly visualize such peaks compared to conventional KMD plots.
Evolving Mass Spectrometry Imaging Analysis
MALDI MS imaging was initially developed to focus on high molecular weight compounds such as proteins and peptides. However, with the expanding applications of MALDI MS imaging, the interests have shifted to include smaller molecules such as lipids, pharmaceuticals, and pharmaceutical metabolites. Conventional MALDI - reflectron TOFMS has difficulty discerning small molecule signals from those of matrix. In the case of MALDI MS imaging, signals from unwanted molecules on the specimen surface will often interfere with signals from the target analytes. High selectivity by means of high mass-resolving power is essential for obtaining reliable target analyte spatial distributions. The SpiralTOFTM-plus with its high mass-resolving power and improved through-put is indispensable for MALDI MS imaging.
Mass Spectrometry Imaging Analysis of Lipids in Mouse Brain Tissue Section
The tissue section specimen was provided by Awazu laboratory, Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University.
MS Imaging of polyethylene glycols having different molecular weight distributions
Analysis of Proteins
For the detailed analysis of a protein, not only the intact protein but also enzymatic digest of the protein can be analyzed. Peptide mass fingerprinting allows identification of a protein.
Mass spectrum of the tryptic digest of bovine serum albumin (BSA) and the results of the peptide mass fingerprinting.
|Amount (fmol)||Number of peptides matched/searched||Sequence coverage (%)||MASCOT score|
Analysis of Small Molecules
Previously, MALDI-TOFMS systems were not suitable for the analysis of small molecules as matrix-derived peaks and continuous chemical noise interfere with the signal from analyte molecules. The SpiralTOF ion optics have solved these problems.