High Sensitivity Analysis of Intact Proteins Using Linear TOF [MALDI Application]

The JMS-S3000 SpiralTOF has a unique 17m flight path that offers the highest resolution MALDI-TOFMS system currently available. However, ions with a very short lifetime or that undergo spontaneous dissociation during their flight cannot be detected by the SpiralTOF (or a conventional reflectron TOF). To address this situation, the SpiralTOF with Linear TOF option can be used for the high sensitivity analysis of intact proteins. In this work, we demonstrate the measurement of intact proteins by using the Linear TOF option for the JEOL SpiralTOF system.